Ace Therapeutics provides H. pylori adhesin research services to help study the structure, adhesion sites, immune response, and potential role of H. pylori adhesins.
Currently, H. pylori adhesins are known to adhere to small molecules (e.g., sugars) and larger ligands (e.g., proteins) of host cells. Some of these adhesins are more clearly studied. CagL interacts with the α5β1, αVβ3, αVβ5, and αVβ6 of host cells. HopQ interacts with CEACAM -1, -3, -5, and -6 of host cells. BabA, HopD, and SabA interact with mucins of host cells by blood group antigens (Leb, A- Leb, and B-Leb), sialyated Lex, and asialyated Lex. In addition, SabA also interacts with sialyated Lex and asialyated Lex of laminin. AlpA and AlpB also bind to laminin. However, the specific adhesion profiles of HorB, HomA/B/C/D, OipA, and HopZ need to be further explored.
Fig. 1 Interaction of H. pylori adhesins with the host cell receptors (Matos et al., 2021)
Ace Therapeutics specializes in H. pylori research and provides research services on adhesins for H. pylori. Our current services include the following categories.
Study of the adhesion sites of adhesins
For adhesins whose specific adhesion sites are currently unknown, such as OipA and HopZ, we offer an adhesion site exploration service. We help to express and purify adhesins with active structures and perform gastric cell adhesion assays to initially explore the adhered molecules in host cells. We can also provide partial amino acid substitution of adhesins to interact with adhered molecules to determine the effective adhesion fragments of adhesins.
Study on the regulation of adhesin expression
The expression of some adhesins is regulated by environmental conditions or their own sequence information. For example, the expression of SabA and HopQ are regulated respectively by pH and salt concentrations, and the expression of HopZ is regulated by the amino acid sequence of SSM. We provide exploration services for adhesins to explore expression regulation by environmental conditions (e.g., pH regulation, salt regulation, and oxidative stress regulation) and their own sequence information.
Study on the adhesion properties of adhesins
The adhesion capacity of adhesins may vary depending on environmental conditions. For example, the adhesion of BabA to gastric cells is influenced by pH. We provide studies on the variation of adhesion capacity with the environment for such adhesins. In addition, we also provide the effect of adhesin gene diversity on adhesion properties.
Study on the immune response and pathogenic effect induced by adhesins
Adhesins, as outer membrane proteins, can induce certain immune responses and cause disease. We offer adhesin overexpression or knockout H. pylori and we offer to perform immunological and pathogenicity studies using them. Furthermore, we help to express and purify adhesins for immunological and pathogenicity studies.
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The colonization of the stomach of H. pylori, the pathogenicity of H. pylori, and the immune-inflammatory response caused by H. pylori are all associated with H. pylori adhesins. Thorough research on H. pylori adhesins can help in developing H. pylori vaccines and finding new targets for drug therapy. Ace Therapeutics provides H. pylori adhesin research services based on the current state of H. pylori adhesin research. If you have a need for these studies, please contact us to assist you.
References
- Bonsor, D. A.; Sundberg, E. J. Roles of adhesion to epithelial cells in gastric colonization by Helicobacter pylori. Helicobacter pylori in Human Diseases. 2019: 57-75.
- Fagoonee, S.; Pellicano, R. Helicobacter pylori: molecular basis for colonization and survival in gastric environment and resistance to antibiotics. A short review. Infectious Diseases. 2019, 51(6): 399-408.
- Matos, R.; et al. Adhesion of Helicobacter species to the human gastric mucosa: A deep look into glycans role. Frontiers in molecular biosciences. 2021, 8: 656439.
※ All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
