Outer membrane vesicles (OMVs) are communication pathways between bacteria to bacteria and between bacteria to hosts. Ace Therapeutics helps to study the structure, composition, function, and influence factors of H. pylori OMVs.
H. pylori OMVs
H. pylori OMVs contain lipopolysaccharides, phosphatidylglycerol, proteins, DNA, and RNA. These components influence its ability to cause disease. For example, the OMVs contain H. pylori virulence proteins, which have a destructive effect on gastric cells. Some proteins and RNA in the OMVs elicit immune responses. What’s more, H. pylori OMVs can also promote biofilm formation, which protects H. pylori. Overall, there is a need for an in-depth study of H. pylori OMVs.
Fig. 1 Virulence factors detected in H. pylori-derived OMVs (Jarzab et al., 2020).
Services overview
Ace Therapeutics helps to study H. pylori OMVs. We offer three types of services to help study H. pylori OMVs.
Effect of environmental and genetic factors on the number and size of OMVs
The number and size of H. pylori OMVs have been found to be related to environmental factors and their genetic profile. We provide services on the effect of different culture additives or other substances (including drugs) on the production of OMVs. We also provide gene (e.g., HP1126) knockout and overexpression services to help explore the number and size of OMVs.
Protein analysis and identification
We can analyze and identify the proteins of the OMVs from different growth stages or different strains. We provide analysis to help find protein differences of the OMVs produced by different growth stages or different strains and we help to investigate the factors of protein differences.
Structure and function studies of the component of H. pylori OMVs
We provide explorations of the functions of unknown proteins, DNAs, and RNAs found in the OMVs of H. pylori. We offer in vitro cellular experiments to explore the effects of the component on the growth, apoptosis, and function of gastric cells. We also offer in vitro animal experiments to explore the immune response and physiological responses induced by the component. For the unknown proteins involved, we also provide structural analysis.
Our strengths
We have a variety of reliable analytical identification instruments (e.g., high-speed cryogenic centrifugal equipment, nanoparticle tracking analysis equipment, and protein structure identification equipment) that can accurately identify the structure and composition of OMVs. Our H. pylori experts have an in-depth knowledge of H. pylori and are well-positioned to analyze the composition of H. pylori OMVs and explore the function of the components and the factors affecting their content.
Collaborate with us
The study of the composition of H. pylori OMVs facilitates the development of H. pylori vaccines. Ace Therapeutics offers services to help study not only the composition of H. pylori OMVs, but also their structure, number, and associated influencing factors. We offer customized services based on specific project needs and we welcome you to collaborate with us on studies related to H. pylori OMVs.
References
- Jarzab, M.; et al. Helicobacter pylori-derived outer membrane vesicles (omvs): Role in bacterial pathogenesis? Microorganisms. 2020, 8(9): 1328.
- Zavan, L.; et al. Helicobacter pylori growth stage determines the size, protein composition, and preferential cargo packaging of outer membrane vesicles. PROTEOMICS. 2019, 19(1-2): 1800209.
※ All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
