The membrane proteins (MPs) of H. pylori (especially adhesins) are essential for colonization, survival, pathogenesis, and cause inflammatory responses. Ace Therapeutics provides research services on H. pylori MPs to help discover vaccine development solutions and drug therapy targets.
The MPs of H. pylori are well worth studying. The MPs of H. pylori play an important role in its survival in the stomach. For example, the adhesion properties of the adhesin BabA vary with pH, which allows H. pylori to survive by adapting to gastric pH changes and adhering to gastric cells at the right time. Then, the MPs of H. pylori are also associated with the pathogenicity of H. pylori and causing inflammatory responses. SabA-positive Western strains of H. pylori can cause intestinal metaplasia, gastric cancer, and corpuscular atrophy. Deletion of AlpA and AlpB in H. pylori East Asian strains reduces IL-8 secretion. What’s more, various pore proteins in the cell membrane of H. pylori have a role in the physiological homeostasis and resistance to antibiotics.
Services overview
Ace Therapeutics offers research services for H. pylori adhesins and other MPs.
Research services for H. pylori adhesins
We offer two services related to the adhesion of H. pylori adhesins. They are adhesion site studies and adhesion properties studies. For adhesion site studies, we can provide partial amino acid substitution of adhesins to find precise adhesion sites. For adhesion properties studies, we mainly provide detection of adhesion properties changing under different environmental conditions. In addition, we also provide studies on the regulated expression of adhesins, the immune response induced by adhesins, and the pathogenic effects of adhesins.
Research services for other MPs
For other MPs, we provide research services for the exploration of H. pylori membrane protein kinds, expression regulation, structure, and functions.
Our strengths
Advanced equipment
We have advanced membrane protein research facilities to study H. pylori MPs, including cryo-electron microscope and high-speed atomic force microscopy (HS-AFM).
Proven research methods
We have specialized expression systems built for H. pylori protein expression, including E. coli, P. pastoris, and L. lactis expression systems. We also have yeast expression systems, insect expression systems, and mammalian expression systems to express human cell MPs to aid in research.
For protein purification, we master enzyme crystallography, affinity chromatography, and ion-exchange chromatography to purify H. pylori MPs and the purified H. pylori MPs are preserved in specially designed phospholipid bilayers with a structure similar to the inner or outer membranes of H. pylori cells.
In the functional research of H. pylori MPs, we master the protein microarray technology, fluorescence resonance energy transfer technology, and gene knockout technology.
Collaborate with us
Ace Therapeutics has in-depth background knowledge of H. pylori, and we have advanced research equipment and reliable experimental methods to achieve a holistic study of H. pylori MPs. In particular, we have developed mature methods in H. pylori adhesin research that can help achieve an efficient and in-depth study of H. pylori adhesins. Collaborate with us! We will be your ideal partner for H. pylori membrane protein research.
References
- Xu, C.; et al. Virulence of Helicobacter pylori outer membrane proteins: an updated review. European Journal of Clinical Microbiology & Infectious Diseases. 2020, 39(10): 1821-30.
- Fagoonee, S.; Pellicano, R. Helicobacter pylori: molecular basis for colonization and survival in gastric environment and resistance to antibiotics. A short review. Infectious Diseases. 2019, 51(6): 399-408.
※ All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
